hcn4 (Alomone Labs)
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hcn4
Hcn4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hcn4/Anti-HCN4+Antibody/10__1038_slash_s41598___026___70409___5-75-6-13
Average 96 stars, based on 195 article reviews
Hcn4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 195 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hcn4/Anti-HCN4+Antibody/10__1038_slash_s41598___026___70409___5-75-6-13
Average 96 stars, based on 195 article reviews
hcn4 - by Bioz Stars,
2026-11
96/100 stars
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Blocking Assay:Article Title: HAND2 invades nucleolar condensates to pioneer lineage-specific cardiac pacemaker gene programs Article Snippet: Samples were blocked with universal blocking buffer (BioGenex) for 10 mins at RT and then incubated with primary antibodies against a-actinin (Sigma A7811), GFP (Invitrogen A11122 or A10262), Hcn4 (Alomone APC-052), Tnnt2 (Thermo MS295P), Med1 (Bethyl A303-876A), Brd4 (Bethyl A301985A50), HP1a (CST 2616), Ubf1 (Thermo PA5-82245e), NPM1 (Thermo 325200), H3K9me3 (CST 13969), H3K27ac (Abcam ab4729), or H3K27me3 (Abcam ab19285) diluted in 0.5X universal blocking buffer overnight at 4 C. The next day, samples were washed three times with 0.1% Triton X-100 and incubated with donkey anti-mouse Alexa Fluor 555 (Invitrogen A-31570), donkey anti-chicken Alexa Fluor 488 (Invitrogen A78948), and donkey anti-rabbit Alexa Fluor 647 (Invitrogen A31573 ) for 1 h at room temperature. .. Samples were blocked with universal blocking buffer (BioGenex) for 10 mins at RT and then incubated with primary antibodies against a-actinin (Sigma A7811), GFP (Invitrogen A11122 or A10262), Article Title: HAND2 invades nucleolar condensates to pioneer lineage-specific cardiac pacemaker gene programs. Article Snippet: .. Samples were blocked with universal blocking buffer (BioGenex) for 10mins at RT and then incubated with primary antibodies against a-actinin (Sigma A7811), GFP (Invitrogen A11122 or A10262), Incubation:Article Title: HAND2 invades nucleolar condensates to pioneer lineage-specific cardiac pacemaker gene programs Article Snippet: Samples were blocked with universal blocking buffer (BioGenex) for 10 mins at RT and then incubated with primary antibodies against a-actinin (Sigma A7811), GFP (Invitrogen A11122 or A10262), Hcn4 (Alomone APC-052), Tnnt2 (Thermo MS295P), Med1 (Bethyl A303-876A), Brd4 (Bethyl A301985A50), HP1a (CST 2616), Ubf1 (Thermo PA5-82245e), NPM1 (Thermo 325200), H3K9me3 (CST 13969), H3K27ac (Abcam ab4729), or H3K27me3 (Abcam ab19285) diluted in 0.5X universal blocking buffer overnight at 4 C. The next day, samples were washed three times with 0.1% Triton X-100 and incubated with donkey anti-mouse Alexa Fluor 555 (Invitrogen A-31570), donkey anti-chicken Alexa Fluor 488 (Invitrogen A78948), and donkey anti-rabbit Alexa Fluor 647 (Invitrogen A31573 ) for 1 h at room temperature. .. Samples were blocked with universal blocking buffer (BioGenex) for 10 mins at RT and then incubated with primary antibodies against a-actinin (Sigma A7811), GFP (Invitrogen A11122 or A10262), Article Title: HAND2 invades nucleolar condensates to pioneer lineage-specific cardiac pacemaker gene programs. Article Snippet: .. Samples were blocked with universal blocking buffer (BioGenex) for 10mins at RT and then incubated with primary antibodies against a-actinin (Sigma A7811), GFP (Invitrogen A11122 or A10262), Article Title: Interleukin-6 Modulates the Expression and Function of HCN Channels: A Link Between Inflammation and Atrial Electrogenesis. Article Snippet: Subsequently, 20 μg of proteins were separated on 4–20% SDS-PAGE (Thermo Fisher Scientific, Carlsbad, CA, USA) and transferred into PVDF membranes (60 min at 398 mA) using standard procedures. .. Blots were incubated overnight at 4 ◦C with a specific antibody against HCN1 (rabbit, #APC-056, Lot APC056AG0740, Alomone Labs, Jerusalem, Israel), HCN2 (rabbit, #APC-030, Lot APC030AG0940, Alomone Labs, Jerusalem, Israel), other:Article Title: Transmitter and ion channel profiles of saccadic omnipause neurons and cholinergic non-omnipause neurons in human nucleus raphe interpositus Article Snippet: HCN4 , Cell-Signaling:Article Title: The HCN4 channel modulates external tufted cell burst firing. Article Snippet: Membrane blots were incubated in TBST buffer with 5% 168 powder milk for 1 h, and then probed in 4 °C overnight with one of the following primary 169 antibodies in same buffer: rabbit anti-HCN1 (#APC-056, 1:200), -HCN2 (#APC-030, 1:200), -170 journals.physiology.org/journal/jn (123.031.253.151) on August 20, 2026 .. HCN3 (#APC-057, 1:200) and - Article Title: Interleukin-6 Modulates the Expression and Function of HCN Channels: A Link Between Inflammation and Atrial Electrogenesis. Article Snippet: Subsequently, 20 μg of proteins were separated on 4–20% SDS-PAGE (Thermo Fisher Scientific, Carlsbad, CA, USA) and transferred into PVDF membranes (60 min at 398 mA) using standard procedures. .. Blots were incubated overnight at 4 ◦C with a specific antibody against HCN1 (rabbit, #APC-056, Lot APC056AG0740, Alomone Labs, Jerusalem, Israel), HCN2 (rabbit, #APC-030, Lot APC030AG0940, Alomone Labs, Jerusalem, Israel), Negative Control:Article Title: Hcn4-deficient zebrafish embryos develop a normal left‒right axis Article Snippet: .. The primary antibody used to label Hcn4 was a |
